PrimePCR™ SYBR® Green Assay: ROPN1B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041400

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038678
Assay Design:   exonic
Chromosome Location:   3:125688104-125688242question
Amplicon Length:   109
Splice Variants Targeted:   ENST00000508088 ENST00000513830 ENST00000504401 ENST00000251776 ENST00000514116 ENST00000310917 ENST00000344408 ENST00000344938 ENST00000405845 ENST00000467907 ENST00000460743 ENST00000496145 ENST00000479867 ENST00000495093 ENST00000484329 ENST00000225893 ENST00000561193 ENST00000560016 ENST00000427275

Gene Information

Description Not Available

Gene Symbol:   ROPN1B
Gene Name:   rhophilin associated tail protein 1B
Aliases:   Not Available
RefSeq:   NC_000003.11 NT_005612.16
Ensembl:   ENSG00000114547
Entrez:   152015
UniGene:   Hs.663128
Chromosome Mapping:   3q21.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 36.400000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download