This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0041393
PrimePCR™ PreAmp for SYBR® Green Assay: HSPA8, Human
PrimePCR™ Template for SYBR® Green Assay: HSPA8, Human
This gene encodes a member of the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group which are also referred to as heat-shock cognate proteins. It functions as a chaperone and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Aug 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.