PrimePCR™ SYBR® Green Assay: NAA10, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041382

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: NAA10, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: NAA10, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038660
Assay Design:   exonic
Chromosome Location:   X:153199842-153200342question
Amplicon Length:   62
Splice Variants Targeted:   ENST00000286794 ENST00000359358 ENST00000358095 ENST00000222823 ENST00000478968 ENST00000394783 ENST00000491606 ENST00000407584 ENST00000266014 ENST00000464845 ENST00000370015 ENST00000393712 ENST00000370009 ENST00000370011 ENST00000432089 ENST00000596770 ENST00000595019 ENST00000599818 ENST00000596061 ENST00000597669 ENST00000595432

Gene Information

N-alpha-acetylation is among the most common post-translational protein modifications in eukaryotic cells. This process involves the transfer of an acetyl group from acetyl-coenzyme A to the alpha-amino group on a nascent polypeptide and is essential for normal cell function. This gene encodes an N-terminal acetyltransferase that functions as the catalytic subunit of the major amino-terminal acetyltransferase A complex. Mutations in this gene are the cause of Ogden syndrome. Alternate splicing results in multiple transcript variants. [provided by RefSeq Jan 2012]

Gene Symbol:   NAA10
Gene Name:   N(alpha)-acetyltransferase 10, NatA catalytic subunit
Aliases:   ARD1, ARD1A, DXS707, MGC71248, TE2
RefSeq:   NC_000023.10 NG_012522.1 NG_013220.1 NT_167198.1
Ensembl:   ENSG00000102030
Entrez:   8260
Chromosome Mapping:   Xq28

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.994900
y-intercept 35.770000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download