PrimePCR™ SYBR® Green Assay: RPLP0, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041375

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Assay Information

Technology:   qPCR
Assay Type   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038653
Assay Design:   exonic
Chromosome Location:   12:120637113-120637205question
Amplicon Length:   63
Splice Variants Targeted:   ENST00000393240 ENST00000543378 ENST00000327564 ENST00000584368 ENST00000341671 ENST00000394713 ENST00000392514 ENST00000551150 ENST00000313104 ENST00000546989 ENST00000546990 ENST00000547211 ENST00000550856 ENST00000547191 ENST00000550423 ENST00000551914 ENST00000228306 ENST00000313401 ENST00000359520 ENST00000367797 ENST00000367796 ENST00000511601 ENST00000260008 ENST00000518665 ENST00000519019 ENST00000520356 ENST00000520727 ENST00000429442 ENST00000377838 ENST00000520214 ENST00000523399

Gene Information

Ribosomes the organelles that catalyze protein synthesis consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein which is the functional equivalent of the E. coli L10 ribosomal protein belongs to the L10P family of ribosomal proteins. It is a neutral phosphoprotein with a C-terminal end that is nearly identical to the C-terminal ends of the acidic ribosomal phosphoproteins P1 and P2. The P0 protein can interact with P1 and P2 to form a pentameric complex consisting of P1 and P2 dimers and a P0 monomer. The protein is located in the cytoplasm. Transcript variants derived from alternative splicing exist; they encode the same protein. As is typical for genes encoding ribosomal proteins there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq Jul 2008]

Gene Symbol:   RPLP0
Gene Name:   ribosomal protein, large, P0
Aliases:   L10E, LP0, MGC111226, MGC88175, P0, PRLP0, RPP0
RefSeq:   NC_000012.11 NT_009775.17
Ensembl:   ENSG00000089157
Entrez:   6175
UniGene:   Hs.546285
Chromosome Mapping   12q24.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.430000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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