PrimePCR™ SYBR® Green Assay: ZNF586, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041369

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ZNF586, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ZNF586, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038647
Assay Design:   exonic
Chromosome Location:   19:58281151-58287912question
Amplicon Length:   67
Splice Variants Targeted:   ENST00000306729 ENST00000306739 ENST00000580534 ENST00000471714 ENST00000284322 ENST00000495063 ENST00000360295 ENST00000540632 ENST00000317338 ENST00000442608 ENST00000335960 ENST00000371586 ENST00000284376 ENST00000438831 ENST00000310118 ENST00000435761 ENST00000439383 ENST00000259470 ENST00000538255 ENST00000308508 ENST00000541476 ENST00000450074 ENST00000262544 ENST00000336714 ENST00000377475 ENST00000377465 ENST00000391702 ENST00000598885 ENST00000598183 ENST00000396154 ENST00000599802 ENST

Gene Information

Description Not Available

Gene Symbol:   ZNF586
Gene Name:   zinc finger protein 586
Aliases:   FLJ20070
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000083828
Entrez:   54807
Chromosome Mapping:   19q13.43

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.620000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download