PrimePCR™ SYBR® Green Assay: NCAPH2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041361

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: NCAPH2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: NCAPH2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038639
Assay Design:   exonic
Chromosome Location:   22:50956170-50956423question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000295633 ENST00000420993 ENST00000395698 ENST00000395701 ENST00000523045 ENST00000299821

Gene Information

This gene encodes one of the non-SMC subunits of the condensin II complex. This complex plays an essential role in mitotic chromosome assembly. Alternate splicing of this gene results in multiple transcript variants.[provided by RefSeq May 2010]

Gene Symbol:   NCAPH2
Gene Name:   non-SMC condensin II complex, subunit H2
Aliases:   CAPH2, MGC15858, MGC18000, MGC2455, MGC4133, MGC5305, MGC8640
RefSeq:   NC_000022.10 NG_016235.1 NT_011526.7 NG_021419.1
Ensembl:   ENSG00000025770
Entrez:   29781
Chromosome Mapping:   22q13.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.620000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download