PrimePCR™ SYBR® Green Assay: IDS, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041358

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038636
Assay Design:   exonic
Chromosome Location:   X:148586565-148586669question
Amplicon Length:   75
Splice Variants Targeted:   ENST00000339561 ENST00000185206 ENST00000202556 ENST00000573081 ENST00000337929 ENST00000564089 ENST00000340951 ENST00000337120 ENST00000519716 ENST00000551649 ENST00000552293 ENST00000550896 ENST00000428389 ENST00000252773 ENST00000288071 ENST00000329125 ENST00000397707 ENST00000560611 ENST00000354181 ENST00000458406 ENST00000397702 ENST00000560164 ENST00000290209 ENST00000558667 ENST00000558589 ENST00000451844 ENST00000507165 ENST00000407472 ENST00000328554 ENST00000405640 ENST00000302641 ENST

Gene Information

Iduronate-2-sulfatase is required for the lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations in this X-chromosome gene that result in enzymatic deficiency lead to the sex-linked Mucopolysaccharidosis Type II also known as Hunter Syndrome. Iduronate-2-sulfatase has a strong sequence similarity with human arylsulfatases A B and C and human glucosamine-6-sulfatase. Multiple alternatively spliced transcript variants that encode different protein isoforms have been described. [provided by RefSeq Oct 2009]

Gene Symbol:   IDS
Gene Name:   iduronate 2-sulfatase
Aliases:   MPS2, SIDS
RefSeq:   NC_000023.10 NG_011900.1 NT_011681.16
Ensembl:   ENSG00000010404
Entrez:   3423
UniGene:   Hs.460960
Chromosome Mapping:   Xq28

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.994900
y-intercept 35.730000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download