PrimePCR™ SYBR® Green Assay: SERPINB10, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041286

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038564
Assay Design:   exonic
Chromosome Location:   18:61582747-61582899question
Amplicon Length:   123
Splice Variants Targeted:   ENST00000397996 ENST00000418725 ENST00000238508

Gene Information

The superfamily of high molecular weight serine proteinase inhibitors (serpins) regulate a diverse set of intracellular and extracellular processes such as complement activation fibrinolysis coagulation cellular differentiation tumor suppression apoptosis and cell migration. Serpins are characterized by a well-conserved tertiary structure that consists of 3 beta sheets and 8 or 9 alpha helices (Huber and Carrell 1989 [PubMed 2690952]). A critical portion of the molecule the reactive center loop connects beta sheets A and C. Protease inhibitor-10 (PI10; SERPINB10) is a member of the ov-serpin subfamily which relative to the archetypal serpin PI1 (MIM 107400) is characterized by a high degree of homology to chicken ovalbumin lack of N- and C-terminal extensions absence of a signal peptide and a serine rather than an asparagine residue at the penultimate position (summary by Bartuski et al. 1997 [PubMed 9268635]).[supplied by OMIM Jan 2010]

Gene Symbol:   SERPINB10
Gene Name:   serpin peptidase inhibitor, clade B (ovalbumin), member 10
Aliases:   PI10, bomapin
RefSeq:   NC_000018.9 NT_025028.14
Ensembl:   ENSG00000242550
Entrez:   5273
UniGene:   Hs.158339
Chromosome Mapping:   18q21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.992000
y-intercept 37.470000
Efficiency 90

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download