PrimePCR™ SYBR® Green Assay: CHRND, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041268

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CHRND, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CHRND, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038546
Assay Design:   exonic
Chromosome Location:   2:233393232-233393352question
Amplicon Length:   91
Splice Variants Targeted:   ENST00000449596 ENST00000258385 ENST00000543200 ENST00000536614 ENST00000457943

Gene Information

The acetylcholine receptor of muscle has 5 subunits of 4 different types: 2 alpha and 1 each of beta gamma and delta subunits. After acetylcholine binding the receptor undergoes an extensive conformation change that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. Defects in this gene are a cause of multiple pterygium syndrome lethal type (MUPSL) congenital myasthenic syndrome slow-channel type (SCCMS) and congenital myasthenic syndrome fast-channel type (FCCMS). Several transcript variants some protein-coding and some not have been found for this gene. [provided by RefSeq Feb 2012]

Gene Symbol:   CHRND
Gene Name:   cholinergic receptor, nicotinic, delta
Aliases:   ACHRD, CMS2A, FCCMS, SCCMS
RefSeq:   NC_000002.11 NG_008028.1 NG_012954.1 NT_005403.17
Ensembl:   ENSG00000135902
Entrez:   1144
Chromosome Mapping:   2q33-q34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.300000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download