PrimePCR™ SYBR® Green Assay: FHL1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041259

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038537
Assay Design:   exonic
Chromosome Location:   X:135288569-135288704question
Amplicon Length:   106
Splice Variants Targeted:   ENST00000370690 ENST00000420362 ENST00000458357 ENST00000452016 ENST00000434885 ENST00000456445 ENST00000449474 ENST00000345434 ENST00000370683 ENST00000370676 ENST00000370674 ENST00000539015 ENST00000535737 ENST00000543669 ENST00000394153 ENST00000394155

Gene Information

This gene encodes a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved tandemly arranged zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. Expression of these family members occurs in a cell- and tissue-specific mode and these proteins are involved in many cellular processes. Mutations in this gene have been found in patients with Emery-Dreifuss muscular dystrophy. Multiple alternately spliced transcript variants which encode different protein isoforms have been described.[provided by RefSeq Nov 2009]

Gene Symbol:   FHL1
Gene Name:   four and a half LIM domains 1
RefSeq:   NC_000023.10 NG_016801.1 NT_011786.16 NG_015895.1
Ensembl:   ENSG00000022267
Entrez:   2273
UniGene:   Hs.435369
Chromosome Mapping:   Xq26

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.390000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download