This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0041249
PrimePCR™ PreAmp for SYBR® Green Assay: POU5F1B, Human
PrimePCR™ Template for SYBR® Green Assay: POU5F1B, Human
This intronless gene was thought to be a transcribed pseudogene of POU class 5 homeobox 1 however it has been reported that this gene can encode a functional protein. The encoded protein is nearly the same length as and highly similar to the POU class 5 homeobox 1 transcription factor has been shown to be a weak transcriptional activator and may play a role in carcinogenesis and eye development. [provided by RefSeq Apr 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.