This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0041232
PrimePCR™ PreAmp for SYBR® Green Assay: SEMA3A, Human
PrimePCR™ Template for SYBR® Green Assay: SEMA3A, Human
This gene is a member of the semaphorin family and encodes a protein with an Ig-like C2-type (immunoglobulin-like) domain a PSI domain and a Sema domain. This secreted protein can function as either a chemorepulsive agent inhibiting axonal outgrowth or as a chemoattractive agent stimulating the growth of apical dendrites. In both cases the protein is vital for normal neuronal pattern development. Increased expression of this protein is associated with schizophrenia and is seen in a variety of human tumor cell lines. Also aberrant release of this protein is associated with the progression of Alzheimer's disease. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.