PrimePCR™ SYBR® Green Assay: FCRLA, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041203

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038481
Assay Design:   exonic
Chromosome Location:   1:161683030-161683165question
Amplicon Length:   106
Splice Variants Targeted:   ENST00000236938 ENST00000367957 ENST00000349527 ENST00000309691 ENST00000294796 ENST00000367953 ENST00000367950 ENST00000367959 ENST00000546024 ENST00000540521 ENST00000367949 ENST00000350710 ENST00000540926

Gene Information

This gene encodes a protein similar to receptors for the Fc fragment of gamma immunoglobulin (IgG). These receptors referred to as FCGRs mediate the destruction of IgG-coated antigens and of cells induced by antibodies. This encoded protein is selectively expressed in B cells and may be involved in their development. This protein may also be involved in the development of lymphomas. Multiple alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. [provided by RefSeq Aug 2011]

Gene Symbol:   FCRLA
Gene Name:   Fc receptor-like A
Aliases:   FCRL, FCRL1, FCRLM1, FCRLX, FCRLb, FCRLc1, FCRLc2, FCRLd, FCRLe, FCRX, FREB, MGC4595
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000132185
Entrez:   84824
UniGene:   Hs.266331
Chromosome Mapping:   1q23.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.890000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download