PrimePCR™ SYBR® Green Assay: LILRA2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041152

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038430
Assay Design:   exonic
Chromosome Location:   19:55086315-55086451question
Amplicon Length:   107
Splice Variants Targeted:   ENST00000391748 ENST00000314446 ENST00000391746 ENST00000391749 ENST00000434421 ENST00000251390 ENST00000391745 ENST00000439534 ENST00000251377 ENST00000251376 ENST00000391737 ENST00000391738 ENST00000396331 ENST00000396327 ENST00000396332 ENST00000427581 ENST00000396317 ENST00000396315 ENST00000396321 ENST00000418536 ENST00000448689 ENST00000324602 ENST00000434867 ENST00000571162 ENST00000575459 ENST00000574138 ENST00000570993 ENST00000571783 ENST00000573976 ENST00000570811 ENST00000577050 ENST

Gene Information

This gene encodes a member of the leukocyte Ig-like receptor (LIR) family of immunoreceptors that are expressed predominantly on monocytes and B cells and at lower levels on dendritic cells and natural killer cells. The LIR family comprises of activating (subfamily A) and inhibitory (subfamily B) cell-surface receptors. The LIR encoded by this gene was shown to be an activating receptor for eosinophils that elicited the release of cytotoxic granule proteins de novo lipid mediator generation and cytokine release through vesicular transport. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Sep 2011]

Gene Symbol:   LILRA2
Gene Name:   leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 2
Aliases:   CD85H, ILT1, LIR-7, LIR7
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000239998
Entrez:   11027
UniGene:   Hs.655593
Chromosome Mapping:   19q13.4

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997600
y-intercept 35.830000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download