This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0041152
PrimePCR™ PreAmp for SYBR® Green Assay: LILRA2, Human
PrimePCR™ Template for SYBR® Green Assay: LILRA2, Human
This gene encodes a member of the leukocyte Ig-like receptor (LIR) family of immunoreceptors that are expressed predominantly on monocytes and B cells and at lower levels on dendritic cells and natural killer cells. The LIR family comprises of activating (subfamily A) and inhibitory (subfamily B) cell-surface receptors. The LIR encoded by this gene was shown to be an activating receptor for eosinophils that elicited the release of cytotoxic granule proteins de novo lipid mediator generation and cytokine release through vesicular transport. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Sep 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.