PrimePCR™ SYBR® Green Assay: WASH4P, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041142

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038420
Assay Design:   exonic
Chromosome Location:   16:64220-64354question
Amplicon Length:   105
Splice Variants Targeted:   ENST00000334554 ENST00000320602 ENST00000405930 ENST00000326592 ENST00000359512 ENST00000285718 ENST00000351293 ENST00000322635 ENST00000423381 ENST00000322680 ENST00000394762 ENST00000370377 ENST00000355149 ENST00000437787 ENST00000296786 ENST00000597984 ENST00000601765 ENST00000600347

Gene Information

Description Not Available

Gene Symbol:   WASH4P
Gene Name:   WAS protein family homolog 4 pseudogene
Aliases:   CXYorf1P, FAM39CP, FLJ31670
RefSeq:   NC_000016.9 NG_003159.3 NT_010393.16
Ensembl:   ENSG00000234769
Entrez:   374677
UniGene:   Not Available
Chromosome Mapping:   16p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998200
y-intercept 35.840000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download