PrimePCR™ SYBR® Green Assay: PSG1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041135

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038413
Assay Design:   exonic
Chromosome Location:   19:43382065-43382238question
Amplicon Length:   144
Splice Variants Targeted:   ENST00000327495 ENST00000595140 ENST00000404209 ENST00000401467 ENST00000306511 ENST00000436291 ENST00000595124 ENST00000595356 ENST00000403380 ENST00000312439 ENST00000244296 ENST00000597058 ENST00000595930 ENST00000320078 ENST00000598133 ENST00000401740 ENST00000406487 ENST00000366175 ENST00000407356 ENST00000407568 ENST00000599812 ENST00000342951 ENST00000404580 ENST00000405312 ENST00000244295 ENST00000599746 ENST00000451895 ENST00000600572 ENST00000433626 ENST00000418820 ENST00000593948 ENST

Gene Information

The human placenta is a multihormonal endocrine organ that produces hormones enzymes and other molecules that support fetal survival and development. Pregnancy-specific beta-1-glycoprotein (PSBG PSG) is a major product of the syncytiotrophoblast reaching concentrations of 100 to 290 mg/l at term in the serum of pregnant women (Horne et al. 1976 [PubMed 971765]). PSG is a member of the immunoglobulin (Ig) superfamily (Watanabe and Chou 1988 [PubMed 3257488]; Streydio et al. 1988 [PubMed 3260773]).[supplied by OMIM Oct 2009]

Gene Symbol:   PSG1
Gene Name:   pregnancy specific beta-1-glycoprotein 1
Aliases:   B1G1, CD66f, DHFRP2, FLJ90598, FLJ90654, PBG1, PSBG1, PSGGA, PSGIIA, SP1
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000231924
Entrez:   5669
UniGene:   Hs.709192
Chromosome Mapping:   19q13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.550000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download