This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0041135
PrimePCR™ PreAmp for SYBR® Green Assay: PSG1, Human
PrimePCR™ Template for SYBR® Green Assay: PSG1, Human
The human placenta is a multihormonal endocrine organ that produces hormones enzymes and other molecules that support fetal survival and development. Pregnancy-specific beta-1-glycoprotein (PSBG PSG) is a major product of the syncytiotrophoblast reaching concentrations of 100 to 290 mg/l at term in the serum of pregnant women (Horne et al. 1976 [PubMed 971765]). PSG is a member of the immunoglobulin (Ig) superfamily (Watanabe and Chou 1988 [PubMed 3257488]; Streydio et al. 1988 [PubMed 3260773]).[supplied by OMIM Oct 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.