PrimePCR™ SYBR® Green Assay: PGA3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041134

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038412
Assay Design:   exonic
Chromosome Location:   11:60977322-60977411question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000533820 ENST00000533129 ENST00000524773 ENST00000279247 ENST00000527323 ENST00000327606 ENST00000266557 ENST00000380516 ENST00000559880 ENST00000536328 ENST00000222250 ENST00000376954 ENST00000338637 ENST00000335012 ENST00000310127 ENST00000539485 ENST00000336451 ENST00000367303 ENST00000494857 ENST00000522656 ENST00000470360 ENST00000004531 ENST00000398090 ENST00000325558 ENST00000543349 ENST00000543505 ENST00000543125 ENST00000372954 ENST00000277465 ENST00000372948 ENST00000372938 ENST

Gene Information

Description Not Available

Gene Symbol:   PGA3
Gene Name:   pepsinogen 3, group I (pepsinogen A)
Aliases:   DKFZp666J2410, PGA4, PGA5
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000229859
Entrez:   643834
UniGene:   Hs.601055
Chromosome Mapping:   11q12.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.900000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download