PrimePCR™ SYBR® Green Assay: COL4A2-AS2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041125

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: COL4A2-AS2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: COL4A2-AS2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038403
Assay Design:   exonic
Chromosome Location:   13:111110461-111110631question
Amplicon Length:   141
Splice Variants Targeted:   ENST00000337774 ENST00000254043 ENST00000340880 ENST00000412718 ENST00000353442 ENST00000415428 ENST00000359422 ENST00000335877 ENST00000333082 ENST00000432854 ENST00000302102 ENST00000545399 ENST00000543770 ENST00000374103 ENST00000419399 ENST00000432695 ENST00000352385 ENST00000310585 ENST00000372977 ENST00000393006 ENST00000378280 ENST00000536991 ENST00000379719 ENST00000373580 ENST00000288815 ENST00000458403 ENST00000371030 ENST00000379410 ENST00000379407 ENST00000379409 ENST00000262715 ENST

Gene Information

Description Not Available

Gene Symbol:   COL4A2-AS2
Gene Name:   COL4A2 antisense RNA 2 (non-protein coding)
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSG00000224821
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.700000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download