PrimePCR™ SYBR® Green Assay: FXYD7, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041115

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038393
Assay Design:   exonic
Chromosome Location:   19:35642157-35642358question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000576903 ENST00000574557 ENST00000586063 ENST00000270310 ENST00000588265 ENST00000409699 ENST00000288745 ENST00000422354 ENST00000395625 ENST00000292599 ENST00000380835

Gene Information

This reference sequence was derived from multiple replicate ESTs and validated by similar human genomic sequence. This gene encodes a member of a family of small membrane proteins that share a 35-amino acid signature sequence domain beginning with the sequence PFXYD and containing 7 invariant and 6 highly conserved amino acids. The approved human gene nomenclature for the family is FXYD-domain containing ion transport regulator. Transmembrane topology has been established for two family members (FXYD1 and FXYD2) with the N-terminus extracellular and the C-terminus on the cytoplasmic side of the membrane. FXYD2 also known as the gamma subunit of the NaK-ATPase regulates the properties of that enzyme. FXYD1 (phospholemman) FXYD2 (gamma) FXYD3 (MAT-8) FXYD4 (CHIF) and FXYD5 (RIC) have been shown to induce channel activity in experimental expression systems. This gene product FXYD7 is novel and has not been characterized as a protein. [RefSeq curation by Kathleen J. Sweadner Ph.D. sweadner@helix.mgh.harvard.edu. Dec 2000]

Gene Symbol:   FXYD7
Gene Name:   FXYD domain containing ion transport regulator 7
Aliases:   FLJ25096
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000221946
Entrez:   53822
UniGene:   Hs.134729
Chromosome Mapping:   19q13.12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997800
y-intercept 36.240000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download