PrimePCR™ SYBR® Green Assay: TMEM110, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041088

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038366
Assay Design:   exonic
Chromosome Location:   3:52883808-52883905question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000342239 ENST00000324109 ENST00000413454 ENST00000596217 ENST00000597723 ENST00000597029 ENST00000352066 ENST00000422090 ENST00000468789 ENST00000436083 ENST00000356987 ENST00000432564 ENST00000263207 ENST00000401994 ENST00000406522 ENST00000406259 ENST00000344269 ENST00000371897 ENST00000371899 ENST00000276816 ENST00000394909 ENST00000252723 ENST00000566936 ENST00000340099 ENST00000046640 ENST00000381870 ENST00000441220 ENST00000414524 ENST00000462833 ENST00000491190 ENST00000309879 ENST

Gene Information

Description Not Available

Gene Symbol:   TMEM110
Gene Name:   transmembrane protein 110
Aliases:   DKFZp667E1121, DKFZp779M0254, FLJ37613, FLJ42395, MGC52022
RefSeq:   NC_000003.11 NT_022517.18
Ensembl:   ENSG00000213533
Entrez:   375346
UniGene:   Hs.730796
Chromosome Mapping:   3p21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.930000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download