PrimePCR™ SYBR® Green Assay: PTPRCAP, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041085

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PTPRCAP, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PTPRCAP, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038363
Assay Design:   exonic
Chromosome Location:   11:67203343-67203540question
Amplicon Length:   168
Splice Variants Targeted:   ENST00000290776 ENST00000535318 ENST00000565874 ENST00000537605 ENST00000564134 ENST00000568666 ENST00000562797 ENST00000425432 ENST00000313565 ENST00000326294 ENST00000338426 ENST00000272845 ENST00000439458

Gene Information

The protein encoded by this gene was identified as a transmembrane phosphoprotein specifically associated with tyrosine phosphatase PTPRC/CD45 a key regulator of T- and B-lymphocyte activation. The interaction with PTPRC may be required for the stable expression of this protein. [provided by RefSeq Jul 2008]

Gene Symbol:   PTPRCAP
Gene Name:   protein tyrosine phosphatase, receptor type, C-associated protein
Aliases:   CD45-AP, LPAP, MGC138602, MGC138603
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000213402
Entrez:   5790
Chromosome Mapping:   11q13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 35.070000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download