PrimePCR™ SYBR® Green Assay: CEMP1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041075

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CEMP1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CEMP1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038353
Assay Design:   exonic
Chromosome Location:   16:2580947-2581092question
Amplicon Length:   116
Splice Variants Targeted:   ENST00000287766 ENST00000295408 ENST00000421804 ENST00000317582 ENST00000355028 ENST00000538149 ENST00000543133 ENST00000556313 ENST00000254976 ENST00000304886 ENST00000302956 ENST00000567119 ENST00000565480 ENST00000382350 ENST00000342272 ENST00000326685 ENST00000309868 ENST00000506113 ENST00000504238 ENST00000508983 ENST00000356541 ENST00000313663 ENST00000414160 ENST00000334126

Gene Information

Description Not Available

Gene Symbol:   CEMP1
Gene Name:   cementum protein 1
Aliases:   CP-23, CP23
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000205923
Entrez:   752014
Chromosome Mapping:   16p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.210000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download