PrimePCR™ SYBR® Green Assay: KRTAP12-3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041069

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: KRTAP12-3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: KRTAP12-3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038347
Assay Design:   exonic
Chromosome Location:   21:46077921-46078016question
Amplicon Length:   66
Splice Variants Targeted:   ENST00000390661 ENST00000383032 ENST00000355162 ENST00000346432 ENST00000376980 ENST00000376946 ENST00000356200 ENST00000378638 ENST00000404249 ENST00000357760 ENST00000358779 ENST00000378633 ENST00000328252 ENST00000445861 ENST00000337827 ENST00000448469 ENST00000553669 ENST00000407149 ENST00000379551 ENST00000505123 ENST00000446965 ENST00000357077 ENST00000264366 ENST00000433179 ENST00000205386 ENST00000418464 ENST00000388781 ENST00000388780 ENST00000414450 ENST00000338950 ENST00000397907 ENST

Gene Information

Description Not Available

Gene Symbol:   KRTAP12-3
Gene Name:   keratin associated protein 12-3
Aliases:   KRTAP12.3
RefSeq:   NC_000021.8 NT_011515.12
Ensembl:   ENSG00000205439
Entrez:   386683
Chromosome Mapping:   21q22.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.150000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download