PrimePCR™ SYBR® Green Assay: MSH5, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041052

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: MSH5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MSH5, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038330
Assay Design:   exonic
Chromosome Location:   6:31730198-31730425question
Amplicon Length:   198
Splice Variants Targeted:   ENST00000395690 ENST00000339553 ENST00000430216 ENST00000414810 ENST00000454820 ENST00000442688 ENST00000458424 ENST00000427687 ENST00000448169 ENST00000437726 ENST00000414514 ENST00000426512 ENST00000444225 ENST00000412630 ENST00000375755 ENST00000375750 ENST00000375703 ENST00000375740 ENST00000395853 ENST00000429846 ENST00000375742 ENST00000534153 ENST00000431848 ENST00000441395 ENST00000441401 ENST00000436192 ENST00000448617 ENST00000436004 ENST00000447504 ENST00000578225 ENST00000427735 ENST

Gene Information

This gene encodes a member of the mutS family of proteins that are involved in DNA mismatch repair and meiotic recombination. This protein is similar to a Saccharomyces cerevisiae protein that participates in segregation fidelity and crossing-over events during meiosis. This protein plays a role in promoting ionizing radiation-induced apoptosis. This protein forms hetero-oligomers with another member of this family mutS homolog 4. Polymorphisms in this gene have been linked to various human diseases including IgA deficiency common variable immunodeficiency and premature ovarian failure. Alternative splicing results multiple transcript variants. Read-through transcription also exists between this gene and the downstream chromosome 6 open reading frame 26 (C6orf26) gene. [provided by RefSeq Feb 2011]

Gene Symbol:   MSH5
Gene Name:   mutS homolog 5 (E. coli)
Aliases:   DKFZp434C1615, G7, MGC2939, MUTSH5, NG23
RefSeq:   NC_000006.11 NT_007592.15 NT_113891.2 NT_167244.1 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1 NT_167249.1 NG_011611.1
Ensembl:   ENSG00000204410
Entrez:   4439
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.920000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download