PrimePCR™ SYBR® Green Assay: XAGE1B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041050

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038328
Assay Design:   exonic
Chromosome Location:   X:52241133-52241248question
Amplicon Length:   86
Splice Variants Targeted:   ENST00000518075 ENST00000375616 ENST00000375613 ENST00000437949 ENST00000399805 ENST00000375600 ENST00000375602 ENST00000399800 ENST00000441417 ENST00000375589 ENST00000375588 ENST00000399795 ENST00000446098 ENST00000375578 ENST00000374959 ENST00000396497 ENST00000438079 ENST00000375567 ENST00000375570 ENST00000429372 ENST00000600138 ENST00000597878 ENST00000597405 ENST00000602068 ENST00000594178 ENST00000596140 ENST00000600638 ENST00000598106 ENST00000597159

Gene Information

This gene is a member of the XAGE subfamily which belongs to the GAGE family. The GAGE genes are expressed in a variety of tumors and in some fetal and reproductive tissues. This gene is strongly expressed in Ewing's sarcoma alveolar rhabdomyosarcoma and normal testis. The protein encoded by this gene contains a nuclear localization signal and shares a sequence similarity with other GAGE/PAGE proteins. Because of the expression pattern and the sequence similarity this protein also belongs to a family of CT (cancer-testis) antigens. Alternative splicing of this gene in addition to alternative transcription start sites results in multiple transcript variants. [provided by RefSeq Jan 2010]

Gene Symbol:   XAGE1B
Gene Name:   X antigen family, member 1B
Aliases:   CT12.1, CT12.1B, CTP9, GAGED2, XAGE1A, XAGE1C, XAGE1D, XAGE1E, XAGE1E.
RefSeq:   NC_000023.10 NT_011638.13
Ensembl:   ENSG00000204382
Entrez:   653220
UniGene:   Not Available
Chromosome Mapping:   Xp11.22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.340000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download