PrimePCR™ SYBR® Green Assay: BTNL2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041047

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038325
Assay Design:   exonic
Chromosome Location:   6:32363919-32364042question
Amplicon Length:   94
Splice Variants Targeted:   ENST00000452747 ENST00000546948 ENST00000550327 ENST00000549560 ENST00000549631 ENST00000457589 ENST00000546419 ENST00000551095 ENST00000548832 ENST00000553032 ENST00000435335 ENST00000546521 ENST00000551471 ENST00000549852 ENST00000549081 ENST00000418649 ENST00000547901 ENST00000552479 ENST00000549943 ENST00000548922 ENST00000458581 ENST00000552394 ENST00000550531 ENST00000551868 ENST00000547502 ENST00000550698 ENST00000551101 ENST00000332539 ENST00000332530 ENST00000451625 ENST00000552523 ENST

Gene Information

Description Not Available

Gene Symbol:   BTNL2
Gene Name:   butyrophilin-like 2 (MHC class II associated)
Aliases:   BTL-II, HSBLMHC1, SS2
RefSeq:   NC_000006.11 NT_007592.15 NT_113891.2 NT_167244.1 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1 NT_167249.1
Ensembl:   ENSG00000204290
Entrez:   56244
UniGene:   Hs.534471
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997900
y-intercept 35.170000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download