PrimePCR™ SYBR® Green Assay: HIST1H2BF, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041014

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038292
Assay Design:   exonic
Chromosome Location:   6:26200043-26200205question
Amplicon Length:   133
Splice Variants Targeted:   ENST00000549491 ENST00000547561 ENST00000552971 ENST00000550101 ENST00000548739 ENST00000553240 ENST00000549836 ENST00000383219 ENST00000443138 ENST00000548637 ENST00000548137 ENST00000359985 ENST00000374708 ENST00000341947 ENST00000357486 ENST00000374714 ENST00000374713 ENST00000395197 ENST00000374712 ENST00000361917 ENST00000448717 ENST00000551758 ENST00000551536 ENST00000552556 ENST00000551786 ENST00000547883 ENST00000551572 ENST00000550998 ENST00000447349 ENST00000549885 ENST00000552765 ENST

Gene Information

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A H2B H3 and H4) form an octamer around which approximately 146 bp of DNA is wrapped in repeating units called nucleosomes. The linker histone H1 interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H2B family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq Jul 2008]

Gene Symbol:   HIST1H2BF
Gene Name:   histone cluster 1, H2bf
Aliases:   H2B/a, H2B/g, H2B/h, H2B/k, H2B/l, H2BFG
RefSeq:   NC_000006.11 NG_001335.1 NT_007592.15
Ensembl:   ENSG00000197846
Entrez:   8343
UniGene:   Hs.182137
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.610000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download