PrimePCR™ SYBR® Green Assay: RPE, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041010

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038288
Assay Design:   exonic
Chromosome Location:   2:210881232-210881330question
Amplicon Length:   69
Splice Variants Targeted:   ENST00000531613 ENST00000283254 ENST00000409101 ENST00000440431 ENST00000360093 ENST00000359429 ENST00000436630 ENST00000408981 ENST00000454822 ENST00000429921 ENST00000453724 ENST00000438265 ENST00000429907 ENST00000441588 ENST00000445268 ENST00000452025 ENST00000438204 ENST00000411934 ENST00000435437 ENST00000540255 ENST00000354506 ENST00000317749 ENST00000326799 ENST00000376016 ENST00000375972 ENST00000452400

Gene Information

Description Not Available

Gene Symbol:   RPE
Gene Name:   ribulose-5-phosphate-3-epimerase
Aliases:   MGC2636, RPE2-1
RefSeq:   NC_000002.11 NT_005403.17
Ensembl:   ENSG00000197713
Entrez:   6120
UniGene:   Hs.282260
Chromosome Mapping:   2q32-q33.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.240000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download