PrimePCR™ SYBR® Green Assay: WDR45, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041004

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038282
Assay Design:   exonic
Chromosome Location:   X:48935700-48957171question
Amplicon Length:   70
Splice Variants Targeted:   ENST00000376372 ENST00000322995 ENST00000356463 ENST00000485908 ENST00000473974 ENST00000376368 ENST00000396681 ENST00000471338 ENST00000475880 ENST00000474053 ENST00000419567 ENST00000476728 ENST00000465382 ENST00000423215 ENST00000553851 ENST00000376358 ENST00000600785 ENST00000602067 ENST00000596615 ENST00000598033 ENST00000601290 ENST00000600730 ENST00000601603 ENST00000602268 ENST00000601175 ENST00000599262 ENST00000601190 ENST00000593749 ENST00000601827 ENST00000596407 ENST00000598900 ENST

Gene Information

This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD) which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes including cell cycle progression signal transduction apoptosis and gene regulation. This gene has a pseudogene at chromosome 4q31.3. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity and full-length nature of some variants have not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   WDR45
Gene Name:   WD repeat domain 45
Aliases:   WDRX1, WIPI-4, WIPI4
RefSeq:   NC_000023.10 NT_079573.4
Ensembl:   ENSG00000196998
Entrez:   11152
UniGene:   Hs.632807
Chromosome Mapping:   Xp11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 35.050000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download