PrimePCR™ SYBR® Green Assay: FAM163B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041003

List Price:    $134.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038281
Assay Design:   exonic
Chromosome Location:   9:136444424-136444514question
Amplicon Length:   61
Splice Variants Targeted:   ENST00000400676 ENST00000537152 ENST00000327787 ENST00000564535 ENST00000568089 ENST00000534366 ENST00000528919 ENST00000398656 ENST00000326134 ENST00000367467 ENST00000261388 ENST00000572599 ENST00000398665 ENST00000440640 ENST00000240328 ENST00000496132 ENST00000356873 ENST00000357164 ENST00000301732 ENST00000382381 ENST00000333850 ENST00000332539 ENST00000311601 ENST00000483233 ENST00000315877 ENST00000035307 ENST00000495645

Gene Information

Description Not Available

Gene Symbol:   FAM163B
Gene Name:   family with sequence similarity 163, member B
Aliases:   C9orf166
RefSeq:   NC_000009.11 NT_035014.4
Ensembl:   ENSG00000196990
Entrez:   642968
UniGene:   Hs.173134
Chromosome Mapping:   9q34.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.840000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download