PrimePCR™ SYBR® Green Assay: CBWD3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041001

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038279
Assay Design:   exonic
Chromosome Location:   9:70862529-70862623question
Amplicon Length:   65
Splice Variants Targeted:   ENST00000259199 ENST00000416503 ENST00000433343 ENST00000356521 ENST00000382447 ENST00000314367 ENST00000377447 ENST00000431099 ENST00000382393 ENST00000382389 ENST00000377400 ENST00000377457 ENST00000377449 ENST00000377441 ENST00000382399 ENST00000382405 ENST00000377395 ENST00000430059 ENST00000429800 ENST00000377384 ENST00000377392 ENST00000449778 ENST00000360171 ENST00000377349 ENST00000377344 ENST00000377342 ENST00000582886 ENST00000578449 ENST00000579515 ENST00000581344 ENST00000577357

Gene Information

Description Not Available

Gene Symbol:   CBWD3
Gene Name:   COBW domain containing 3
Aliases:   FLJ60409, bA561O23.1
RefSeq:   NC_000009.11 NT_008470.19
Ensembl:   ENSG00000196873
Entrez:   445571
UniGene:   Not Available
Chromosome Mapping:   9q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998300
y-intercept 35.370000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download