PrimePCR™ SYBR® Green Assay: FAM72A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040998

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: FAM72A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: FAM72A, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038276
Assay Design:   exonic
Chromosome Location:   1:206145481-206153985question
Amplicon Length:   96
Splice Variants Targeted:   ENST00000369392 ENST00000369390 ENST00000452190 ENST00000355228 ENST00000400889 ENST00000369175 ENST00000367129 ENST00000367128 ENST00000341209 ENST00000583781 ENST00000582310 ENST00000580350 ENST00000578606 ENST00000583602 ENST00000584006 ENST00000584774 ENST00000584486 ENST00000581557

Gene Information

Description Not Available

Gene Symbol:   FAM72A
Gene Name:   family with sequence similarity 72, member A
Aliases:   MGC169168, MGC182081, MGC182095, MGC46651, MGC57827
RefSeq:   NC_000001.10 NT_086602.2
Ensembl:   ENSG00000196550
Entrez:   729533
Chromosome Mapping:   1q32.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.570000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download