PrimePCR™ SYBR® Green Assay: NACA, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040997

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: NACA, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: NACA, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038275
Assay Design:   exonic
Chromosome Location:   12:57107345-57108160question
Amplicon Length:   107
Splice Variants Targeted:   ENST00000521764 ENST00000550920 ENST00000550952 ENST00000356769 ENST00000552540 ENST00000393891 ENST00000548563 ENST00000546392 ENST00000549259 ENST00000552055 ENST00000546862 ENST00000549855 ENST00000454682

Gene Information

The protein encoded by this gene associates with basic transcription factor 3 (BTF3) to form the nascent polypeptide-associated complex (NAC). NAC binds to nascent proteins as they emerge from the ribosome blocking interaction with the signal recognition particle (SRP) and preventing mistranslocation to the endoplasmic reticulum. However nascent proteins with an exposed signal peptide will not be bound by the encoded protein enabling them to bind the SRP and enter the secretory pathway. This protein has been determined to be an IgE autoantigen in atopic dermatitis patients. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq Oct 2011]

Gene Symbol:   NACA
Gene Name:   nascent polypeptide-associated complex alpha subunit
Aliases:   MGC117224, NACA1
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000196531
Entrez:   4666
Chromosome Mapping:   12q23-q24.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.880000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download