PrimePCR™ SYBR® Green Assay: KRTAP10-6, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040982

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038260
Assay Design:   exonic
Chromosome Location:   21:46011306-46011442question
Amplicon Length:   107
Splice Variants Targeted:   ENST00000483722 ENST00000356303 ENST00000594030 ENST00000352410 ENST00000391620 ENST00000400368 ENST00000380102 ENST00000397911 ENST00000380095 ENST00000334670 ENST00000495514 ENST00000273395 ENST00000355385 ENST00000423517 ENST00000275358

Gene Information

Description Not Available

Gene Symbol:   KRTAP10-6
Gene Name:   keratin associated protein 10-6
Aliases:   KAP10.6, KAP18.6, KRTAP18-6, KRTAP18.6
RefSeq:   NC_000021.8 NT_011515.12
Ensembl:   ENSG00000188155
Entrez:   386674
UniGene:   Hs.689666
Chromosome Mapping:   21q22.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.920000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download