PrimePCR™ SYBR® Green Assay: FCAR, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040969

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038247
Assay Design:   exonic
Chromosome Location:   19:55396776-55396874question
Amplicon Length:   69
Splice Variants Targeted:   ENST00000392782 ENST00000392783 ENST00000541068 ENST00000355831 ENST00000343439 ENST00000391726 ENST00000355524 ENST00000391725 ENST00000345937 ENST00000359272 ENST00000391723 ENST00000391724 ENST00000433231 ENST00000571891 ENST00000572552 ENST00000576568 ENST00000575913 ENST00000575866 ENST00000576509 ENST00000573084 ENST00000572443 ENST00000574618 ENST00000571746 ENST00000576265 ENST00000573433 ENST00000574058 ENST00000575074 ENST00000573273 ENST00000574738

Gene Information

This gene is a member of the immunoglobulin gene superfamily and encodes a receptor for the Fc region of IgA. The receptor is a transmembrane glycoprotein present on the surface of myeloid lineage cells such as neutrophils monocytes macrophages and eosinophils where it mediates immunologic responses to pathogens. It interacts with IgA-opsonized targets and triggers several immunologic defense processes including phagocytosis antibody-dependent cell-mediated cytotoxicity and stimulation of the release of inflammatory mediators. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   FCAR
Gene Name:   Fc fragment of IgA, receptor for
Aliases:   CD89
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000186431
Entrez:   2204
UniGene:   Hs.659872
Chromosome Mapping:   19q13.42

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.390000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download