PrimePCR™ SYBR® Green Assay: POLR1D, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040967

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038245
Assay Design:   exonic
Chromosome Location:   13:28196024-28196135question
Amplicon Length:   82
Splice Variants Targeted:   ENST00000380948 ENST00000286096 ENST00000302979 ENST00000399697 ENST00000562377 ENST00000296487 ENST00000268711 ENST00000536708 ENST00000338399 ENST00000260947 ENST00000449967 ENST00000375664 ENST00000375646 ENST00000347934 ENST00000354911 ENST00000220676

Gene Information

The protein encoded by this gene is a component of the RNA polymerase I and RNA polymerase III complexes which function in the synthesis of ribosomal RNA precursors and small RNAs respectively. Mutations in this gene are a cause of Treacher Collins syndrome (TCS) a craniofacial development disorder. Alternative splicing results in multiple transcript variants. [provided by RefSeq Apr 2011]

Gene Symbol:   POLR1D
Gene Name:   polymerase (RNA) I polypeptide D, 16kDa
Aliases:   AC19, FLJ20616, MGC9850, POLR1C, RPA16, RPA9, RPAC2, RPC16, RPO1-3, TCS2
RefSeq:   NC_000013.10 NT_024524.14
Ensembl:   ENSG00000186184
Entrez:   51082
UniGene:   Hs.507584
Chromosome Mapping:   13q12.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.730000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download