PrimePCR™ SYBR® Green Assay: FAM120C, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040955

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: FAM120C, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: FAM120C, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038233
Assay Design:   exonic
Chromosome Location:   X:54209026-54209148question
Amplicon Length:   93
Splice Variants Targeted:   ENST00000566854 ENST00000444799 ENST00000375180 ENST00000328235 ENST00000477084 ENST00000513291 ENST00000601768 ENST00000424107 ENST00000558583 ENST00000391696 ENST00000339673 ENST00000282479 ENST00000594687 ENST00000600333 ENST00000601479

Gene Information

This gene encodes a potential transmembrane protein and lies in a region where mutations and deletions have been associated with intellectual disability and autism. Alternative splicing results in multiple transcript variants. [provided by RefSeq Aug 2011]

Gene Symbol:   FAM120C
Gene Name:   family with sequence similarity 120C
Aliases:   CXorf17, FLJ20506, FLJ43676, ORF34
RefSeq:   NC_000023.10 NG_021316.1 NT_011630.14
Ensembl:   ENSG00000184083
Entrez:   54954
Chromosome Mapping:   Xp11.22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.740000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download