PrimePCR™ SYBR® Green Assay: MTCP1NB, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040941

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: MTCP1NB, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MTCP1NB, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038219
Assay Design:   exonic
Chromosome Location:   X:154289973-154290122question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000361999 ENST00000355123 ENST00000406921 ENST00000412011 ENST00000380868 ENST00000306077 ENST00000526986 ENST00000585101 ENST00000474627 ENST00000369484 ENST00000369479 ENST00000373829 ENST00000538820 ENST00000336356 ENST00000340308 ENST00000447932 ENST00000326232 ENST00000469193 ENST00000429996 ENST00000598989 ENST00000599805

Gene Information

This gene was identified by involvement in some t(X;14) translocations associated with mature T-cell proliferations. This region has a complex gene structure with a common promoter and 5' exon spliced to two different sets of 3' exons that encode two different proteins. This gene represents the downstream 8 kDa protein that localizes to mitochondria.[provided by RefSeq Mar 2009]

Gene Symbol:   MTCP1NB
Gene Name:   mature T-cell proliferation 1 neighbor
Aliases:   C6.1B, MGC2069, MTCP1, p8, p8MTCP1
RefSeq:   NC_000023.10 NG_005114.1 NT_167198.1
Ensembl:   ENSG00000182712
Entrez:   100272147
Chromosome Mapping:   Xq28

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.320000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download