PrimePCR™ SYBR® Green Assay: KIAA0174, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040937

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: KIAA0174, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: KIAA0174, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038215
Assay Design:   exonic
Chromosome Location:   16:71950444-71950534question
Amplicon Length:   61
Splice Variants Targeted:   ENST00000455752 ENST00000539006 ENST00000535270 ENST00000320574 ENST00000434396 ENST00000298694 ENST00000298693 ENST00000397682 ENST00000291691 ENST00000445935 ENST00000397685 ENST00000397906 ENST00000373471 ENST00000329517 ENST00000403325 ENST00000405009 ENST00000229239 ENST00000396856 ENST00000396861 ENST00000396859 ENST00000396858 ENST00000568581 ENST00000544564 ENST00000541571 ENST00000378799 ENST00000537613 ENST00000424485 ENST00000329908 ENST00000541918 ENST00000534994 ENST00000378798 ENST

Gene Information

Description Not Available

Gene Symbol:   KIAA0174
Gene Name:   KIAA0174
Aliases:   IST1, MGC117220
RefSeq:   NC_000016.9 NT_010498.15
Ensembl:   ENSG00000182149
Entrez:   9798
Chromosome Mapping:   16q22.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 35.970000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download