PrimePCR™ SYBR® Green Assay: KRTAP9-7, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040930

List Price:    $134.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038208
Assay Design:   exonic
Chromosome Location:   17:39432274-39432429question
Amplicon Length:   126
Splice Variants Targeted:   ENST00000455970 ENST00000377721 ENST00000254072 ENST00000334109 ENST00000394008 ENST00000391354 ENST00000571104 ENST00000570861 ENST00000570825 ENST00000573531 ENST00000381153 ENST00000456145 ENST00000564831 ENST00000328423 ENST00000431282 ENST00000393701 ENST00000568461 ENST00000288040 ENST00000449317 ENST00000440411 ENST00000257931 ENST00000548043 ENST00000425394 ENST00000541793 ENST00000314151 ENST00000569347 ENST00000536907 ENST00000254337 ENST00000495880 ENST00000296483 ENST00000358743 ENST

Gene Information

Description Not Available

Gene Symbol:   KRTAP9-7
Gene Name:   keratin associated protein 9-7
Aliases:   KAP9.7
RefSeq:   Not Available
Ensembl:   ENSG00000180386
Entrez:   85277
UniGene:   Not Available
Chromosome Mapping:   17q12-q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 35.620000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download