PrimePCR™ SYBR® Green Assay: ZSCAN2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040918

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038196
Assay Design:   exonic
Chromosome Location:   15:85147126-85147237question
Amplicon Length:   82
Splice Variants Targeted:   ENST00000371191 ENST00000403491 ENST00000371185 ENST00000371187 ENST00000407417 ENST00000371189 ENST00000361677 ENST00000404826 ENST00000389531 ENST00000312541 ENST00000429381 ENST00000269919 ENST00000540936 ENST00000448803 ENST00000546275 ENST00000546148 ENST00000442073 ENST00000334141 ENST00000502939 ENST00000379358 ENST00000327179 ENST00000541040 ENST00000538076 ENST00000485222 ENST00000488625 ENST00000542879 ENST00000320985 ENST00000559858 ENST00000560721 ENST00000437844 ENST00000542815 ENST

Gene Information

The protein encoded by this gene contains several copies of zinc finger motif which is commonly found in transcriptional regulatory proteins. Studies in mice show that this gene is expressed during embryonic development and specifically in the testis in adult mice suggesting that it may play a role in regulating genes in germ cells. Alternative splicing of this gene results in several transcript variants encoding different isoforms. [provided by RefSeq Jul 2008]

Gene Symbol:   ZSCAN2
Gene Name:   zinc finger and SCAN domain containing 2
Aliases:   FLJ20595, ZFP29, ZNF854
RefSeq:   NC_000015.9 NT_010274.17
Ensembl:   ENSG00000176371
Entrez:   54993
UniGene:   Hs.594023
Chromosome Mapping:   15q25.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.490000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download