This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0040918
PrimePCR™ PreAmp for SYBR® Green Assay: ZSCAN2, Human
PrimePCR™ Template for SYBR® Green Assay: ZSCAN2, Human
The protein encoded by this gene contains several copies of zinc finger motif which is commonly found in transcriptional regulatory proteins. Studies in mice show that this gene is expressed during embryonic development and specifically in the testis in adult mice suggesting that it may play a role in regulating genes in germ cells. Alternative splicing of this gene results in several transcript variants encoding different isoforms. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.