PrimePCR™ SYBR® Green Assay: C1QTNF4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040894

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038171
Assay Design:   exonic
Chromosome Location:   11:47611407-47611496question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000302514 ENST00000530097 ENST00000409052 ENST00000259206 ENST00000437265 ENST00000421349 ENST00000553172 ENST00000382545 ENST00000215882 ENST00000379919 ENST00000412585 ENST00000436729 ENST00000435618 ENST00000546599 ENST00000242159 ENST00000245457 ENST00000274979 ENST00000402430 ENST00000355481 ENST00000359596 ENST00000360985 ENST00000300482 ENST00000397928 ENST00000397932 ENST00000369875 ENST00000433628 ENST00000457502 ENST00000369878 ENST00000538373 ENST00000399410 ENST00000572882 ENST

Gene Information

Description Not Available

Gene Symbol:   C1QTNF4
Gene Name:   C1q and tumor necrosis factor related protein 4
Aliases:   CTRP4, ZACRP4
RefSeq:   NC_000011.9 NT_009237.18
Ensembl:   ENSG00000172247
Entrez:   114900
UniGene:   Hs.662633
Chromosome Mapping:   11q11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.780000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download