PrimePCR™ SYBR® Green Assay: LCE1D, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040893

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038170
Assay Design:   exonic
Chromosome Location:   1:152770249-152770410question
Amplicon Length:   132
Splice Variants Targeted:   ENST00000517434 ENST00000331343 ENST00000270720 ENST00000314100 ENST00000481247 ENST00000377837 ENST00000377836 ENST00000436129 ENST00000252996 ENST00000379288 ENST00000327367 ENST00000414749 ENST00000429400 ENST00000313375 ENST00000354613 ENST00000434326 ENST00000395189 ENST00000326233 ENST00000360090 ENST00000368766 ENST00000533131 ENST00000416441 ENST00000543033 ENST00000308893 ENST00000574203 ENST00000573014 ENST00000574926 ENST00000570829 ENST00000575492 ENST00000572858

Gene Information

Description Not Available

Gene Symbol:   LCE1D
Gene Name:   late cornified envelope 1D
Aliases:   LEP4
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000172155
Entrez:   353134
UniGene:   Hs.490235
Chromosome Mapping:   1q21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.630000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download