PrimePCR™ SYBR® Green Assay: LGALS9B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040879

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: LGALS9B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: LGALS9B, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038156
Assay Design:   exonic
Chromosome Location:   17:20361583-20363683question
Amplicon Length:   103
Splice Variants Targeted:   ENST00000395473 ENST00000302228 ENST00000313648 ENST00000577392 ENST00000584661 ENST00000310394 ENST00000581545 ENST00000582333 ENST00000328114 ENST00000583322 ENST00000584941 ENST00000412421 ENST00000423676 ENST00000324290

Gene Information

This gene was initially thought to represent a pseudogene of galectin 9; however this transcript has good exon-intron structure and encodes a predicted protein of the same size as and highly similar to galectin 9. This gene is one of two similar loci on chromosome 17p similar to galectin 9 and now thought to be protein-encoding. This gene is the more centromeric gene. [provided by RefSeq Jul 2008]

Gene Symbol:   LGALS9B
Gene Name:   lectin, galactoside-binding, soluble, 9B
Aliases:   FLJ58146, FLJ75081
RefSeq:   NC_000017.10 NT_010718.16
Ensembl:   ENSG00000170298
Entrez:   284194
Chromosome Mapping:   17p11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.050000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download