PrimePCR™ SYBR® Green Assay: HINT1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040873

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: HINT1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: HINT1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038150
Assay Design:   exonic
Chromosome Location:   5:130500874-130500968question
Amplicon Length:   65
Splice Variants Targeted:   ENST00000338662 ENST00000373913 ENST00000401952 ENST00000011684 ENST00000536531 ENST00000396988 ENST00000256852 ENST00000304043 ENST00000508488 ENST00000506908 ENST00000513012 ENST00000294016 ENST00000572288 ENST00000375771 ENST00000375766 ENST00000400773 ENST00000281722 ENST00000510397 ENST00000313005 ENST00000528053 ENST00000594949 ENST00000597268

Gene Information

Description Not Available

Gene Symbol:   HINT1
Gene Name:   histidine triad nucleotide binding protein 1
Aliases:   FLJ30414, FLJ32340, HINT, PKCI-1, PRKCNH1
RefSeq:   NC_000005.9 NT_034772.6
Ensembl:   ENSG00000169567
Entrez:   3094
Chromosome Mapping:   5q31.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.130000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download