PrimePCR™ SYBR® Green Assay: CPT1C, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040871

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CPT1C, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CPT1C, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038148
Assay Design:   exonic
Chromosome Location:   19:50195496-50195644question
Amplicon Length:   119
Splice Variants Targeted:   ENST00000332314 ENST00000216044 ENST00000354199 ENST00000595969 ENST00000594587 ENST00000602019 ENST00000405931 ENST00000598293 ENST00000598396 ENST00000392518 ENST00000323446 ENST00000373610 ENST00000373609 ENST00000231484 ENST00000256413 ENST00000382998 ENST00000373980 ENST00000344683 ENST00000537502 ENST00000376592 ENST00000376590 ENST00000376583 ENST00000242351 ENST00000464606 ENST00000471652 ENST00000405995 ENST00000357304 ENST00000458550 ENST00000372249 ENST00000166244

Gene Information

This gene encodes a member of the carnitine/choline acetyltransferase family. The encoded protein regulates the beta-oxidation and transport of long-chain fatty acids into mitochondria and may play a role in the regulation of feeding behavior and whole-body energy homeostasis. Alternatively spliced transcript variants encoding multiple protein isoforms have been observed for this gene. [provided by RefSeq Dec 2010]

Gene Symbol:   CPT1C
Gene Name:   carnitine palmitoyltransferase 1C
Aliases:   CATL1, CPT1-B, CPT1P, CPTI-B, CPTIC, FLJ23809
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000169169
Entrez:   126129
Chromosome Mapping:   19q13.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998300
y-intercept 35.290000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download