PrimePCR™ SYBR® Green Assay: MECP2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040870

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038147
Assay Design:   exonic
Chromosome Location:   X:153297894-153363075question
Amplicon Length:   99
Splice Variants Targeted:   ENST00000318217 ENST00000394649 ENST00000376954 ENST00000338637 ENST00000199320 ENST00000506390 ENST00000395715 ENST00000519674 ENST00000303391 ENST00000453960 ENST00000407218 ENST00000415944 ENST00000593953 ENST00000599945 ENST00000601699 ENST00000598049 ENST00000593590 ENST00000601709

Gene Information

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2 MBD1 MBD2 MBD3 and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins with the exception of MBD3 is capable of binding specifically to methylated DNA. MECP2 MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. [provided by RefSeq Jul 2009]

Gene Symbol:   MECP2
Gene Name:   methyl CpG binding protein 2 (Rett syndrome)
Aliases:   AUTSX3, DKFZp686A24160, MRX16, MRX79, MRXS13, MRXSL, PPMX, RS, RTS, RTT
RefSeq:   NC_000023.10 NT_167198.1 NG_007107.1 NG_008387.1
Ensembl:   ENSG00000169057
Entrez:   4204
UniGene:   Hs.731393
Chromosome Mapping:   Xq28

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.120000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download