PrimePCR™ SYBR® Green Assay: MLKL, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040865

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: MLKL, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MLKL, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038142
Assay Design:   exonic
Chromosome Location:   16:74729418-74729625question
Amplicon Length:   178
Splice Variants Targeted:   ENST00000298808 ENST00000307518 ENST00000455090 ENST00000370655 ENST00000587435 ENST00000587544 ENST00000336861 ENST00000246635 ENST00000374796 ENST00000359003 ENST00000306247 ENST00000308807 ENST00000573267 ENST00000575686

Gene Information

Description Not Available

Gene Symbol:   MLKL
Gene Name:   mixed lineage kinase domain-like
Aliases:   FLJ34389
RefSeq:   NC_000016.9 NT_010498.15
Ensembl:   ENSG00000168404
Entrez:   197259
Chromosome Mapping:   16q23.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996000
y-intercept 36.630000
Efficiency 89

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download