PrimePCR™ SYBR® Green Assay: HIST1H2BI, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040864

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: HIST1H2BI, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: HIST1H2BI, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038141
Assay Design:   exonic
Chromosome Location:   6:26273309-26273422question
Amplicon Length:   84
Splice Variants Targeted:   ENST00000289316 ENST00000377777 ENST00000356530 ENST00000359985 ENST00000244601 ENST00000356350 ENST00000377733 ENST00000356950 ENST00000396891 ENST00000377401 ENST00000359465 ENST00000449538 ENST00000396980 ENST00000303806 ENST00000369167 ENST00000545683 ENST00000427880 ENST00000369155 ENST00000369160 ENST00000578867 ENST00000584281 ENST00000583773 ENST00000583061

Gene Information

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A H2B H3 and H4) form an octamer around which approximately 146 bp of DNA is wrapped in repeating units called nucleosomes. The linker histone H1 interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H2B family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq Jul 2008]

Gene Symbol:   HIST1H2BI
Gene Name:   histone cluster 1, H2bi
Aliases:   H2B/a, H2B/g, H2B/h, H2B/k, H2B/l, H2BFK
RefSeq:   NC_000006.11 NG_001335.1 NT_007592.15
Ensembl:   ENSG00000168242
Entrez:   8346
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.890000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download