PrimePCR™ SYBR® Green Assay: MFSD3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040859

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: MFSD3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MFSD3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038136
Assay Design:   exonic
Chromosome Location:   8:145736424-145736527question
Amplicon Length:   74
Splice Variants Targeted:   ENST00000316664 ENST00000392400 ENST00000428187 ENST00000333602 ENST00000381916 ENST00000527972 ENST00000457912 ENST00000399154 ENST00000301327 ENST00000378493 ENST00000378501 ENST00000414679 ENST00000334005 ENST00000378473 ENST00000278655 ENST00000421422 ENST00000559234 ENST00000373263 ENST00000540170 ENST00000591471 ENST00000544914 ENST00000392153 ENST00000324411 ENST00000541583 ENST00000268893 ENST00000355701 ENST00000258173 ENST00000398114 ENST00000590706 ENST00000307650 ENST00000263382

Gene Information

Description Not Available

Gene Symbol:   MFSD3
Gene Name:   major facilitator superfamily domain containing 3
Aliases:   Not Available
RefSeq:   NC_000008.10 NG_015828.1 NG_016430.1 NT_037704.5
Ensembl:   ENSG00000167700
Entrez:   113655
Chromosome Mapping:   8q24.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998300
y-intercept 36.250000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download